Micturition-related neural activity depends on the location, cell-type, and projection pathway; revealed by two-photon calcium imaging in the anterior cingulate cortex and the primary motor cortex.

Shimura H1, Manita S2, Mochizuki T1, Kira S1, Sawada N1, Kitamura K2, Mitsui T1

Research Type

Pure and Applied Science / Translational

Abstract Category

Research Methods / Techniques

Abstract 153
Best Pure and Applied Science
Scientific Podium Session 15
Thursday 24th October 2024
12:30 - 12:45
Hall N102
Basic Science Imaging Animal Study Voiding Dysfunction
1. University of Yamanashi Graduate School of Medical Sciences, Department of Urology, 2. University of Yamanashi Graduate School of Medical Sciences, Department of Neuroscience
Presenter
Links

Abstract

Hypothesis / aims of study
The mechanism of micturition is complicated and especially in the cerebral cortex, micturition-related neural activity remains poorly understood. Two-photon calcium imaging enables to observe individual neural activities with high spatial resolution. In the present study, we observed the neural activities in the cerebral cortex using two-photon calcium imaging and evaluated the relationship with micturition. Our purpose was to reveal the ratio and the localization of micturition-related neurons, and the features of neural activity in various cell-type patterns.
Study design, materials and methods
We genetically expressed red calcium indicators (jRGECO1a) in the anterior cingulate cortex (ACC) or the primary motor cortex (M1) of mice. Two-photon calcium imaging from the ACC or the M1 was performed with bladder perfusion under urethane anesthesia. Micturition-related neurons were extracted according to neural synchrony with micturition (Figure1A).
Cell-type patterns were A) non-selective neurons, B) layer 5 pyramidal neurons, and C) certain projection neurons; the ACC to the periaqueductal gray matter (PAG) or the M1 to the pontine micturition center (PMC) (n=3 or 4 in each group, total n = 22) (Figure 1B).
Results
Micturition-related neural activity was individually identified in every region and pattern (Figure 2A). The rates of micturition-related neurons per all observed neurons were 1) 6.34%, 2) 10.97%, 3) 9.42% in the ACC and 1) 6.61%, 2) 10.33%, 3) 8.50% in the M1.
The hot spot (high density region) of micturition-related neurons in the ACC (posterior and deep region) were more local than in the M1 (Figure 2B). The peak timing histogram of B) layer 5 pyramidal neural activities in the ACC was bimodal (the former mean; 0.72sec, the latter mean; 11.53sec), and the latter peak was analogous with histogram of C) the ACC-PAG projection neural activities (mean 10.75sec). Furthermore, the population of the delayed neurons was located in the hot spot of the ACC (posterior and deep region). On the other hand, the patterns of neural activity were uniform among each variation in the M1 (Figure 2C).
Interpretation of results
The number of micturition-related neurons is not so large population both in the ACC and the M1. ACC may have multi-functional clusters regarding micturition, depending on their location, cell-type, and projection pathway. On the other hand, M1 may have single functional cluster well synchronized with bladder pressure.
Concluding message
We represented that micturition-related neural activities in the cerebral cortex could be detected individually, which was the first study using two-photon calcium imaging for micturition. The various neural activity timings would indicate that these neurons play different roles for micturition each other. Utilizing this calcium imaging method would uncover the mechanism of micturition in the future.
Figure 1 Figure 1
Figure 2 Figure 2
Disclosures
Funding Reseach founding:JSPS KAKENHI Grant Number 20K18135, JP22K09496, Grant for Young Researcher from Yamanashi Prefecture, Young Research 108 Grant from the Japanese Urological Association and GSK Japan Research Grant 2021 Clinical Trial No Subjects Animal Species mouse Ethics Committee the Animal Experiment Committees of University of Yamanashi (#A27-1)
Citation

Continence 12S (2024) 101495
DOI: 10.1016/j.cont.2024.101495

10/11/2024 21:35:07