In geriatric gynecologic fields, inflammation is a common health problem in postmenopausal women. It can cause many symptoms as itching, dryness, pain, obesity, urinary symptoms etc. It makes to decrease the quality of life and to affect a negative effect on gynecologic symptoms.  This study aims to evaluate the change of inflammation using Light emitting diode (LED) therapy in mice as a preclinical study.

We performed a prospective evaluation of 20 postmenopausal mouses (control group; n=10, LED group; n=10) which were undergone bilateral ovariectomy from July 2021 to September 2021.  We used to the mixed wavelengths of three types as the 460-nm LED (blue), the 592-nm LED (amber), the 630-nm LED (red). Each mouse got LED device (Bellalux®, Linkoptics, Gwangu, Korea) on its buttock for 20 minutes for 2weeks. We applied candida albicans (ATCC, 11006, University Boulevard Manassas, VA, 20110, USA) to mice’s buttock and the blood sample in heart of mouse and analyzed to cell blood count (CBC), C-reactive protein (CRP) analysis. Our study was approved by the experimental animal institutional review board under registration number CKU-02-2021-004. Data were analyzed using SPSS software (version 22; IBM Corp., Armonk, NY, USA).  Statistical significance was considered as P< 0.05). The paired t-test analysis was analyzed to compare between no LED group and LED therapy group for 2 weeks after LED treatment.

We compared to the result of blood test between no LED group (n=10) and LED therapy group (n=10) for 2 weeks after LED treatment.  The mice of treatment group were treated on LED devices on its buttock for 20 minutes for 2 weeks. On blood analysis, mean whole blood cell (WBC) count of no therapy group was 4.37 ± 1.03 *103 cells/μL to be decreased to 3.12 ± 0.76 *103 cells/μL of the LED therapy group (p=0.007). The lymphocyte count was changed from 3.90 ± 0.90*103 cells/μL to 2.73 ± 0.68*103 cells/μL after LED therapy (p=0.004). There is no statistically significant change in CRP of both groups.

Even though our experiment is the  pre-clinical stage, LED therapy improved the WBC count and lymphocyte count as a inflammatory marker in mice of both groups. LED can be a treatment option to systemic inflammation in postmenopause.

It is expected that postmenopausal women would opt for adequate treatment option to restore in gynecologic symptoms related to inflammation. In the absence of clinical data on postmenopausal women, this provides evidence for a future approach.