Long-Term Effect of Local and Systemic Adipose Tissue-Derived Stem Cells on Stress Urinary Incontinence in Rats

Elsharabasy A1, Elkashef A2, Barakat N2, Khater S2, Awadalla A3, Hussein A4, Gomaa M1, Abdel-Maboud M1, Shokeir A3

Research Type

Pure and Applied Science / Translational

Abstract Category

Research Methods / Techniques

Abstract 534
On Demand Research Methods / Techniques
Scientific Open Discussion Session 35
On-Demand
Basic Science Animal Study Stress Urinary Incontinence Stem Cells / Tissue Engineering
1. Department of General Surgery, Faculty of Veterinary Medicine, Zagazig University, Egypt, 2. Department of Urology, Urology and Nephrology Center, Mansoura University, Egypt, 3. Center of Excellence for Genome and Cancer Research, Urology and Nephrology Center, Mansoura University, Egypt, 4. Department of Physiology, Faculty of Medicine, Mansoura University, Egypt
Presenter
Links

Abstract

Hypothesis / aims of study
Stress urinary incontinence (SUI) is a major health problem that has many social and economic impacts, so we evaluated the long-term effect of locally and systemically administrated adipose tissue-derived mesenchymal stem cells (ADMSCs) on the treatment of SUI-induced in a rat model.
Study design, materials and methods
Thirty-two female Sprague-Dawley rats were randomly allocated into 4 groups (8 rats each); control, SUI, local ADMSCs and systemic ADMSCs groups. SUI was induced by vaginal dilatation of the rats via inflating the balloon of a urethral catheter with 5 mL sterile saline in the vagina for 8 hours. Rats then received 0.9% saline, local ADMSCs treatment (periurethal injection at 5 and 7 o’clock positions of the vesico-urethral junction) and systemic ADMSCs treatment (injection in the tail vein) in SUI, local and systemic ADMSCs groups, respectively for 4 weeks. Urodynamic study in terms of abdominal leak point pressure (ALPP), threshold pressure (TP) and bladder capacity (BC) was performed. Rats were then sacrificed and their periurethral regions were sent for routine histopathological examination.
Results
There was significant improvement in the results of the urodynamic study in terms of ALPP, TP and BC after administration of either local or systemic ADMSCs for 4 weeks relative to SUI group (Figure 1). There was also significant evidence of regeneration of the muscle layers in the periurethral regions with less inflammation and fibrosis in local and systemic ADMSCs groups compared to SUI group (Figure 2). Apart from BC, the locally administrated ADMSCs significantly provided more favorable effects than the systemically administrated ADMSCs.
Interpretation of results
SUI resulted in significant damage of the muscle layers in the periurethral regions with subsequent inflammation and fibrosis. SUI group showed significant decrease in the urodynamic parameters; ALPP, TP and BC compared to the control group. However, ADMSCs administration significantly regenerated the affected muscle layers in the periurethral regions after induction of SUI. The routine histopathological examination of the periurethral regions revealed less inflammation and less fibrosis in both local and systemic ADMSCs groups relative to SUI group. Local and systemic administration of ADMSCs for 4 weeks also showed significant improvement in the results of the urodynamic study in terms of ALPP, TP and BC compared to SUI group. Apart from BC, local administration of ADMSCs offered more significant improvement in the morphological and urodynamic dysfunctions than the systemic administration of ADMSCs.
Concluding message
Long-term administration of ADMSCs either locally or systemically in the periurethral regions in SUI improves the morphological and urodynamic dysfunctions of the affected urethral sphincters.
Figure 1 Figure 1: Urodynamic changes after local and systemic administration of ADMSCs for the treatment of SUI-induced in rats for 4 weeks.
Figure 2 Figure 2: Routine histopathological examination of the urethra.
Disclosures
Funding None Clinical Trial No Subjects Animal Species Rat
13/11/2024 01:02:34